Name :
KLF4 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
KLF4 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HT-1080 cells, mouse testis tissue, NCCIT cell, NIH/3T3 cells, RAW264.7 cells. Positive IF detected in human embronic stem cells. Positive IHC detected in human colon tissue, human spleen tissue, human tonsil tissue. Observed molecular weight by Western-blot: 50-60 kDa
Tested applications :
ELISA, IHC, IP, IF, WB
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
EZF antibody; GKLF antibody; KLF4 antibody; Krueppel like factor 4 antibody; Kruppel like factor 4 (gut) antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of KLF4 recombinant protein (Accession Number: BC030811). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:20-1:200
Background :
The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa.
References :
Li W, Liu M, Su Y, Zhou X, Liu Y, Zhang X. The Janus-faced roles of Krüppel-like factor 4 in oral squamous cell carcinoma cells. Oncotarget. 6(42):44480-44494. 2015. Yin F, Lan R, Zhang X. LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16. Molecular and cellular biology. 34(2):158-79. 2014. Zhang X, Lu F, Wang J. Pluripotent stem cell protein Sox2 confers sensitivity to LSD1 inhibition in cancer cells. Cell reports. 5(2):445-57. 2013. Shan T, Zhou C, Yang R. Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β-catenin signaling. Cell biology international. 39(1):35-43. 2015. Zheng Y, Zou F, Wang J. Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein. The Journal of biological chemistry. 290(14):8975-86. 2015. Kissing S, Hermsen C, Repnik U. Vacuolar ATPase in phagosome-lysosome fusion. The Journal of biological chemistry. 290(22):14166-80. 2015. Stavri S, Simionescu M, Kardassis D, Gafencu AV. Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages. Biochemical and biophysical research communications. 468(1-2):66-72. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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