Name :
LIG4 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
LIG4 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human testis tissue. Positive WB detected in mouse testis tissue, HeLa cells, HepG2 cells, mouse liver tissue, rat testis tissue. Observed molecular weight by Western-blot: 100-104 kDa
Tested applications :
ELISA, WB, IHC
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
DNA ligase 4 antibody; DNA ligase IV antibody; LIG4 antibody; ligase IV antibody; DNA antibody; ATP dependent antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of LIG4 recombinant protein (Accession Number: NM_002312). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IHC: 1:20-1:200
Background :
Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4. Read more about this antibody on the blog: http://blog.ptglab.com/index.php/proteintechs-anti-neil3-antibody-used-investigate-mechanisms-of-dna-repair/?preview=true
References :
Paran N, De Silva FS, Senkevich TG, Moss B. Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication. Cell host & microbe. 6(6):563-9. 2009. Cousin W, Ho ML, Desai R. Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage. PloS one. 8(5):e63528. 2013. Xing M, Yang M, Huo W. Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nature communications. 6:6233. 2015. Dong H, Shi Q, Song X. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Toxicology and applied pharmacology. 286(1):10-6. 2015. Guo C, Nakazawa Y, Woodbine L. XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency. The Journal of allergy and clinical immunology. 136(4):1007-17. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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