Name :
MAD2L2 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MAD2L2 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A375 cells, fetal human brain tissue, HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 24kd
Tested applications :
ELISA, WB, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
hREV7 antibody; MAD2 like protein 2 antibody; MAD2B antibody; MAD2L2 antibody; REV7 antibody; REV7 homolog antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MAD2L2 recombinant protein (Accession Number: NM_001127325). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000
Background :
MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediates the second polymerase switching in translation DNA synthesis by mediating the inteaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.
References :
Sharma S, Hicks JK, Chute CL. REV1 and polymerase ζ facilitate homologous recombination repair. Nucleic acids research. 40(2):682-91. 2012. Listovsky T, Sale JE. Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset. The Journal of cell biology. 203(1):87-100. 2013. Tomida J, Takata K, Lange SS. REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ. Nucleic acids research. 43(2):1000-11. 2015. Kim MS, Machida Y, Vashisht AA, Wohlschlegel JA, Pang YP, Machida YJ. Regulation of error-prone translesion synthesis by Spartan/C1orf124. Nucleic acids research. 41(3):1661-8. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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