Name :
MCM6 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MCM6 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, HeLa cells, Jurkat cells, mouse ovary tissue, mouse spleen tissue, mouse thymus tissue. Positive IP detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 105 kDa
Tested applications :
ELISA, WB, IP, IHC, IF
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
MCG40308 antibody; MCM6 antibody; Mis5 antibody; P105MCM antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MCM6 recombinant protein (Accession Number: NM_005915). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:50-1:500
Background :
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be ‘DNA licensing factors’ which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3′ to 5′ DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027].
References :
Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014. Yuan Q, Han J, Cong W. Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity. International journal of nanomedicine. 9:4829-46. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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