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Name :
Anti-Calreticulin antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
Rabbit polyclonal to Calreticulin

Tested applications :
ELISA, WB, IHC-P, IP

Species reactivity :
Human Calreticulin

Alternative names :
Calr antibody; CALR antibody; Calregulin antibody; calregulin antibody; Calreticulin antibody; Calreticulin antibody; calreticulin) antibody; cC1qR antibody; CRP55 antibody; CRP55 antibody; CRT antibody; CRT antibody; endoplasmic reticulum resident protein 60 antibody; endoplasmic reticulum resident protein 60 antibody; ERp60 antibody; ERp60 antibody; grp60 antibody; HACBP antibody; HACBP antibody; RO antibody; Sicca syndrome antigen A (autoantigen Ro antibody; SSA antibody; RO antibody; CRT antibody; SSA antibody; cC1qR antibody; HEL-S-99n antibody; CRT antibody; Calregulin antibody

Immunogen :
Human Calreticulin (His Tag) recombinant protein

Isotype :
Rabbit IgG

Preparation :
Produced in rabbits immunized with purified, recombinant Human Calreticulin (rh Calreticulin; P27797; Met1-Ala413). Calreticulin specific IgG was purified by Human Calreticulin affinity chromatography.

Clonality :
Polyclonal

Formulation :
0.2 μm filtered solution in PBS

Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications :
WB: 2-10 μg/mlELISA:0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Calreticulin. The detection limit for Human Calreticulin is IHC-P: 0.05-1 μg/mlIP: 1-4 μg/mg of lysate

Background :
Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.

References :
Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7. Chao MP, et al. (2010) Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2(63):63ra94. Andrin, C, et al. (1998) Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules. Biochemistry. 37(29):10386-94.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel