Name :
MDH1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MDH1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in HL-60 cells, HepG2 cells, human kidney tissue, human spleen tissue, mouse liver tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human renal cell carcinoma tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 36 kDa
Tested applications :
ELISA, WB, IF, FC, IP, IHC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
Cytosolic malate dehydrogenase antibody; MDH s antibody; MDH1 antibody; MDHA antibody; MOR2 antibody
Immunogen :
Human MDH1 Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MDH1 recombinant protein (Accession Number: NM_005917). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:50-1:500IF: 1:10-1:100
Background :
MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743).
References :
Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM. An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Cell. 162(3):540-51. 2015. Dai J, Xu W, Zhao X. Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa. Reproduction (Cambridge, England). 151(3):227-37. 2016. Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014. Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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