Name :
MGMT antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MGMT Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, MCF7 cells. Positive IHC detected in human kidney tissue, human placenta tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 22 kDa
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human; other species not tested.
Alternative names :
MGMT antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MGMT recombinant protein (Accession Number: XM_005252682). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]
References :
Duan H, He Z, Ma J. Global and MGMT promoter hypomethylation independently associated with genomic instability of lymphocytes in subjects exposed to high-dose polycyclic aromatic hydrocarbon. Archives of toxicology. 87(11):2013-22. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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