Name :
MMP1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MMP1 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human cervical cancer tissue, rat testis tissue. Positive WB detected in A549 cells, A375 cells, HepG2 cells, Jurkat cells, mouse skeletal muscle tissue, NIH/3T3 cells, rat skin tissue. Positive IP detected in A549 cells. Observed molecular weight by Western-blot: 52-56 kDa,62 kDa
Tested applications :
ELISA, WB, IF, IP, FC, IHC
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
CLG antibody; CLGN antibody; Fibroblast collagenase antibody; Interstitial collagenase antibody; Matrix metalloproteinase 1 antibody; MMP 1 antibody; MMP1 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MMP1 recombinant protein (Accession Number: NM_002421). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).
References :
Wu J, Wu G, Lv L. MicroRNA-34a inhibits migration and invasion of colon cancer cells via targeting to Fra-1. Carcinogenesis. 33(3):519-28. 2012. Ding Q, Zhong H, Qi Y. Anti-arthritic effects of crocin in interleukin-1β-treated articular chondrocytes and cartilage in a rabbit osteoarthritic model. Inflammation research : official journal of the European Histamine Research Society … Zhang M, Liu Y, Feng H. CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. The American journal of pathology. 182(2):565-76. 2013. Ding QH, Cheng Y, Chen WP, Zhong HM, Wang XH. Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes. European journal of pharmacology. 708(1-3):1-7. 2013. Wu DQ, Zhong HM, Ding QH, Ba L. Protective effects of biochanin A on articular cartilage: in vitro and in vivo studies. BMC complementary and alternative medicine. 14:444. 2014. Kmiecik AM, Pula B, Suchanski J. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression. PloS one. 10(5):e0124865. 2015. Li T, Niu L, Li M. Effects of small interfering RNA-mediated downregulation of the Krüppel-like factor 4 gene on collagen metabolism in human hepatic stellate cells. Molecular medicine reports. 12(3):3972-8. 2015. Zhang X, Han X, Yin L. Potent effects of dioscin against liver fibrosis. Scientific reports. 5:9713. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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