Name :
MMP9 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MMP9 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human cervical cancer tissue, human stomach cancer tissue. Positive WB detected in Jurkat cells, HepG2 cells, mouse heart tissue, NIH/3T3 cells, Raji cells, rat brain tissue, zebrafish tissue. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 67 kDa, 92 kDa
Tested applications :
ELISA, WB, IHC, IP, IF
Species reactivity :
Human, Mouse, Rat, Zebrafish; other species not tested.
Alternative names :
92 kDa gelatinase antibody; 92 kDa type IV collagenase antibody; CLG4B antibody; Gelatinase B antibody; GELB antibody; Matrix metalloproteinase 9 antibody; MMP 9 antibody; MMP9 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MMP9 recombinant protein (Accession Number: NM_004994). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:1000IHC: 1:20-1:200IF: 1:20-1:200
Background :
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP’s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades type IV and V collagens. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa and it can be detected a processed form of 68 kDa or 82 kDa and this protein can exsit as a dimer of 180 kDa(PMID:7492685).
References :
Sakashita N, Motooka Y, Suganuma M. A case of pulmonary capillary hemangiomatosis with pulmonary fibrosis associated with MMP-9 related pulmonary remodeling. Pathology international. 61(5):306-12. 2011. Xu Z, Liu D, Fan C, Luan L, Zhang X, Wang E. DIXDC1 increases the invasion and migration ability of non-small-cell lung cancer cells via the PI3K-AKT/AP-1 pathway. Molecular carcinogenesis. 53(11):917-25. 2014. Zhang S, Qi Y, Xu Y. Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation. Neurochemistry international. 63(5):522-32. 2013. Xiang M, Su H, Hu Y, Hu Y, Yang T, Shu G. Chemical composition of total flavonoids from Salvia chinensia Benth and their pro-apoptotic effect on hepatocellular carcinoma cells: potential roles of suppressing cellular NF-κB signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:420-6. 2013. Li X, Huang T, Jiang G, Gong W, Qian H, Zou C. Proteasome inhibitor MG132 enhances TRAIL-induced apoptosis and inhibits invasion of human osteosarcoma OS732 cells. Biochemical and biophysical research communications. 439(2):179-86. 2013. Yi Y, Wu H, Gao Q. Interferon regulatory factor (IRF)-1 and IRF-2 are associated with prognosis and tumor invasion in HCC. Annals of surgical oncology. 20(1):267-76. 2013. Liu N, Li Y, Su S, Wang N, Wang H, Li J. Inhibition of cell migration by ouabain in the A549 human lung cancer cell line. Oncology letters. 6(2):475-479. 2013. Sang H, Li T, Li H, Liu J. Down-regulation of Gab1 inhibits cell proliferation and migration in hilar cholangiocarcinoma. PloS one. 8(11):e81347. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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